1 Tween 20 PBS for 1 hour and incubated with primary antibodies specific for keratin 5 Abcam, ER Novocastra, and Ki 67 Dako overnight at 4 C
priligy pills The cells were harvested, cellular RNA was extracted using an RNeasy Mini kit QIAGEN, Valencia, CA, USA in an RNase free environment according to the manufacturer s instructions, and it was subsequently purified by ethanol precipitation